6.151 Microfabrication Project Laboratory, Fall 2003

 

Integrating PCR amplification with silicon field-effect

sensors for real-time DNA detection

Instructors: Professors Scott Manalis and Martin Schmidt
Guest Instructor: Dr. Raj Chakrabarti
Teaching Assistant: Maxim Shusteff
Advisors: Peter Russo and Dr. Paul Benning
Prerequisites: 6.152 or equivalent
 
FINAL REPORT ........ HEATER MOVIE
ASSIGNMENTS READING TIMELINE PRESENTATIONS
LAB NOTEBOOK MEMBERS   RESOURCES
 
     

 










 

1998: Kopp et al. micromachined a chemical amplifier to perform polymerase chain reaction (PCR) in continuous flow at high speed. (Science 280 1046)

COURSE DESCRIPTION: The project goal for fall 2003 is to integrate PCR, silicon field-effect sensors, and microfluidics for applications that require real-time and point-of-use DNA analysis. In achieving this goal, students will gain first-hand experience with MEMS design, process development, fabrication in the Microsystems Technology Laboratory and device characterization as well as biochemical methods relevant for performing PCR. This course will require ~15 hours per week. Please pre-register by emailing your academic/research background and course schedule for fall 2003 to: scottm@media.mit.edu.

 

     


2002: Fritz et al. reported the selective and real-time detection of label-free DNA using a silicon field-effect detector. (PNAS 99 14142) 2002: The 6.151 class successfully integrated PDMS microfluidics with planar silicon field-effect sensors.